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- Binu Timsina, Pavel Kindlmann, Maan B Rokaya, Naděžda Vrchotová, Jan Tříska, Štěpán Horník, and Jan Sýkora.
- Institute for Environmental Studies, Faculty of Science, Charles University, Benátská 2, 128 01 Prague, Czech Republic. binu.timsina@gmail.com.
- Molecules. 2018 May 3; 23 (5).
AbstractThe medicinal plant Swertia multicaulis D. Don was collected in Rasuwa District (Nepal) and the xanthone content of its ethyl acetate extracts was studied. The total amount of xanthones in S. multicaulis determined by HPLC reaches almost 13 g of xanthones per 1 kg of dry matter. The identification of xanthones in S. multicaulis was achieved by a combination of HPLC, LC⁻MS and LC⁻NMR. The final assignment of the individual chemical structures was provided by NMR, supported by preparative HPLC. In eight chromatographic peaks, four major xanthones were identified—1,3-dihydroxy-5,8-dimethoxyxanthone, 1-hydroxy-3,5,8-trimethoxyxanthone, bellidifolin (1,5,8-tri-hydroxy-3-methoxyxanthone), and decussatin (1-hydroxy-3,7,8-trimethoxyxanthone).
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