• J. Clin. Virol. · Nov 2020

    Use of a simplified sample processing step without RNA extraction for direct SARS-CoV-2 RT-PCR detection.

    • Ruby Barza, Parul Patel, Linda Sabatini, and Kamaljit Singh.
    • NorthShore University HealthSystem, Department of Pathology and Laboratory Medicine, Evanston, IL, USA.
    • J. Clin. Virol. 2020 Nov 1; 132: 104587.

    AbstractThe severe acute respiratory syndrome coronavirus (SARS-CoV-2) pandemic has resulted in significant shortages of RT-PCR testing supplies including RNA extraction kits. The goal of our study was to determine if a simplified heat-RNA release method would provide comparable detection of SARS-CoV-2 without the need for nucleic acid extraction. RT-PCR results using the ChromaCode HDPCR™ SARS-CoV-2 were compared using the heat-RNA release method and an automated RNA extraction system (EMAG). The heat-RNA release method correctly identified 94 % (81/86 nasopharyngeal samples) that were positive for SARS-CoV-2. Five samples that were missed by heat-RNA release method had a mean Ct value: 35 using the automated extraction instrument, indicating a very low viral load. Our findings show that a simple heat-RNA release method is a reasonable alternative for the majority of COVID-19 positive patients and can help overcome the cost and availability issues of RNA extraction reagents.Copyright © 2020 Elsevier B.V. All rights reserved.

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