• Appl. Environ. Microbiol. · Nov 2001

    Poly(3-hydroxybutyrate) synthesis genes in Azotobacter sp. strain FA8.

    • M J Pettinari, G J Vázquez, D Silberschmidt, B Rehm, A Steinbüchel, and B S Méndez.
    • Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
    • Appl. Environ. Microbiol. 2001 Nov 1; 67 (11): 5331-4.

    AbstractGenes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for beta-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced into Ralstonia eutropha PHB-4 (phbC-negative mutant), and the recombinant accumulated PHB when either glucose or octanoate was used as a source of carbon, indicating that this PHB synthase cannot incorporate medium-chain-length hydroxyalkanoates into PHB.

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