• Pancreas · Sep 2019

    Inflammation Promotes Progression of Pancreatic Cancer Through WNT/β-Catenin Pathway-Dependent Manner.

    • Rui Ren, Jie Yu, Yan Zhang, Sheng-Fei Wang, Xia Guo, Meng Shen, Meng-Dan Xu, Min Jiang, Qiaoming Zhi, Kai Chen, Min Tao, Meng-Yao Wu, Dong-Mei Gu, and Wei Li.
    • From the Department of Oncology, the First Affiliated Hospital of Soochow University.
    • Pancreas. 2019 Sep 1; 48 (8): 1003-1014.

    ObjectiveIdentify the molecular mechanism of inflammatory stimuli induced pancreatic cancer progression.MethodsRNA-seq, microarray assay and bioinformatics analyses were used to identify differentially expressed genes. Immunohistochemical staining was performed to evaluate CD68, CD163, β-catenin, CD103, CCL3 markers. Quantitative real-time polymerase chain reaction (qRT-PCR), luciferase reporter assay, apoptosis assay, wound healing assay and immunofluorescence were performed to study the relationship of inflammatory stimuli and WNT/β-catenin pathway.ResultsDifferentially expressed genes of macrophage-conditioned medium-treated pancreatic cancer cells were related with WNT/β-catenin pathway. Inflammatory stimuli could activate WNT/β-catenin signaling pathway. In 106 pancreatic cancer patients, nuclear β-catenin expression of CD68-high group was much higher than CD68-low group (P < 0.05), as same as CD163 (P < 0.05). Inflammatory stimuli downregulated the expression of CCL3 via WNT/β-catenin pathway and inhibited the chemotaxis of CD103 dendritic cells. Six pancreatic cancer prognosis associating genes were upregulated by inflammatory stimuli via WNT/β-catenin pathway. Transforming growth factor-β promoted malignant biological behavior of pancreatic cancer cells through WNT/β-catenin pathway-dependent mechanism.ConclusionsOur present study provided a novel mechanism involved in the inflammation-driven cancer progression through tumor immune escape and downstream gene regulation of WNT/β-catenin pathway-dependent manner.

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