• Ryan T Fellers, Joseph B Greer, Bryan P Early, Xiang Yu, Richard D LeDuc, Neil L Kelleher, and Paul M Thomas.
• Department of Chemistry, Department of Molecular Biosciences, Proteomics Center of Excellence, Northwestern, University, Evanston, IL, USA.
• Proteomics. 2015 Apr 1; 15 (7): 1235-8.

AbstractMany top-down proteomics experiments focus on identifying and localizing PTMs and other potential sources of “mass shift” on a known protein sequence. A simple application to match ion masses and facilitate the iterative hypothesis testing of PTM presence and location would assist with the data analysis in these experiments. ProSight Lite is a free software tool for matching a single candidate sequence against a set of mass spectrometric observations. Fixed or variable modifications, including both PTMs and a select number of glycosylations, can be applied to the amino acid sequence. The application reports multiple scores and a matching fragment list. Fragmentation maps can be exported for publication in either portable network graphic (PNG) or scalable vector graphic (SVG) format. ProSight Lite can be freely downloaded from http://prosightlite.northwestern.edu, installs and updates from the web, and requires Windows 7 or a higher version.

13 keywords...

hide…