• Pamela Reyes-Ortega, Ragu VarmanDurairajDDepartamento de Neurobiología Celular y Molecular. Instituto de Neurobiología. Universidad Nacional Autónoma de México, Campus Juriquilla. Boulevard Juriquilla #3001. Juriquilla, Querétaro; CP 76230, México; Department of Pharmacology , Verónica M Rodríguez, and Daniel Reyes-Haro.
• Departamento de Neurobiología Celular y Molecular. Instituto de Neurobiología. Universidad Nacional Autónoma de México, Campus Juriquilla. Boulevard Juriquilla #3001. Juriquilla, Querétaro; CP 76230, México.
• Behav. Brain Res. 2020 Aug 17; 392: 112606.

AbstractDehydration-Induced Anorexia (DIA) is a murine model that reproduces weight loss and avoidance of food, despite its availability. The prefrontal cortex (PFC) integrates sensory inputs and updates associative learning to promote (hunger) or inhibit (satiety) food-seeking behavior. In this study we tested if anorexia induces a pro-inflammatory environment associated with microglia in the medial prefrontal cortex (mPFC) and orbitofrontal cortex (OFC), specific subregions of the PFC involved in appetite. Our results showed that anorexia increased microglial density, promoted a de-ramified morphology and augmented the de-ramified/ramified ratio in the mPFC and OFC but not in the motor cortex. Anorexia also increased the expression of the pro-inflammatory cytokines TNF-α, IL-6 and IL-1β. This pro-inflammatory environment associated with microglia activation correlates with neuronal damage as revealed by Fluoro Jade C (FJC) and NeuN immunolabeling. We conclude that anorexia triggers a pro-inflammatory environment associated with microglia that correlates with neurodegeneration in the mPFC and OFC.Copyright © 2020 Elsevier B.V. All rights reserved.

6 keywords...

hide…