• Sci. Total Environ. · Nov 2020

    First detection of SARS-CoV-2 RNA in wastewater in North America: A study in Louisiana, USA.

    • Samendra P Sherchan, Shalina Shahin, Lauren M Ward, Sarmila Tandukar, Tiong G Aw, Bradley Schmitz, Warish Ahmed, and Masaaki Kitajima.
    • Department of Environmental Health Sciences, Tulane University, 1440 Canal Street, Suite 2100, New Orleans, LA 70112, USA. Electronic address: sshercha@tulane.edu.
    • Sci. Total Environ. 2020 Nov 15; 743: 140621.

    AbstractWe investigated the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater samples in southern Louisiana, USA. Untreated and treated wastewater samples were collected on five occasions over a four-month period from January to April 2020. The wastewater samples were concentrated via ultrafiltration (Method A), and an adsorption-elution method using electronegative membranes (Method B). SARS-CoV-2 RNA was detected in 2 out of 15 wastewater samples using two reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays (CDC N1 and N2). None of the secondary treated and final effluent samples tested positive for SARS-CoV-2 RNA. To our knowledge, this is the first study reporting the detection of SARS-CoV-2 RNA in wastewater in North America, including the USA. However, concentration methods and RT-qPCR assays need to be refined and validated to increase the sensitivity of SARS-CoV-2 RNA detection in wastewater.Copyright © 2020 Elsevier B.V. All rights reserved.

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