• Cell · Jun 2021

    Low-dose Ad26.COV2.S protection against SARS-CoV-2 challenge in rhesus macaques.

    • Xuan He, Abishek Chandrashekar, Roland Zahn, Frank Wegmann, Jingyou Yu, Noe B Mercado, Katherine McMahan, Amanda J Martinot, Cesar Piedra-Mora, Sidney Beecy, Sarah Ducat, Ronnie Chamanza, Sietske Rosendahl Huber, Marjolein van Heerden, Leslie van der Fits, Erica N Borducchi, Michelle Lifton, Jinyan Liu, Felix Nampanya, Shivani Patel, Lauren Peter, Lisa H Tostanoski, Laurent Pessaint, Alex Van Ry, Brad Finneyfrock, Jason Velasco, Elyse Teow, Renita Brown, Anthony Cook, Hanne Andersen, Mark G Lewis, Hanneke Schuitemaker, and Dan H Barouch.
    • Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
    • Cell. 2021 Jun 24; 184 (13): 3467-3473.e11.

    AbstractWe previously reported that a single immunization with an adenovirus serotype 26 (Ad26)-vector-based vaccine expressing an optimized SARS-CoV-2 spike (Ad26.COV2.S) protected rhesus macaques against SARS-CoV-2 challenge. To evaluate reduced doses of Ad26.COV2.S, 30 rhesus macaques were immunized once with 1 × 1011, 5 × 1010, 1.125 × 1010, or 2 × 109 viral particles (vp) Ad26.COV2.S or sham and were challenged with SARS-CoV-2. Vaccine doses as low as 2 × 109 vp provided robust protection in bronchoalveolar lavage, whereas doses of 1.125 × 1010 vp were required for protection in nasal swabs. Activated memory B cells and binding or neutralizing antibody titers following vaccination correlated with protective efficacy. At suboptimal vaccine doses, viral breakthrough was observed but did not show enhancement of disease. These data demonstrate that a single immunization with relatively low dose of Ad26.COV2.S effectively protected against SARS-CoV-2 challenge in rhesus macaques, although a higher vaccine dose may be required for protection in the upper respiratory tract.Copyright © 2021 Elsevier Inc. All rights reserved.

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