• Laith Al-Eitan, Fouad Abdelaziz Almomani, Sohaib Mahmoud Al-Khatib, Hanan Abdulraheem Aljamal, Mohammed Nayef Al-Qusami, and Rawan Abdulraheem Aljamal.
• From the Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, Irbid, Jordan.
• Ann Saudi Med. 2021 Jul 1; 41 (4): 206-215.

BackgroundHelicobacter pylori infection is widespread, affecting about 50% of the global population. Polymorphisms in host genes such as the toll-like receptor 4 (TLR4) might affect the susceptibility and severity of infection and treatment success.ObjectiveInvestigate the susceptibility and severity of H pylori infection with host TLR4 (rs11536889, rs4986790, rs200109652, rs10759932), TLR5 (rs5744174, rs2072493, rs746250566), TLR10 (rs559182335, rs10004195) polymorphisms.DesignAnalytical, cross-sectional.SettingEndoscopy clinic at tertiary care center.Patients And MethodsGenomic DNA was extracted from formalin-fixed paraffin-embedded tissues collected from H pylori-infected patients and healthy individuals. The single nucleotide polymorphisms (SNPs) within the targeted TLR genes were genotyped to assess the genetic association of various SNPs with disease severity.Main Outcome MeasuresEffect of genotype distribution on H pylori infection.Sample Size250 peptic ulcer patients and 217 controls.ResultsThe TLR10 genotype showed no significant association with H pylori infection except for rs10004195 (T>A) (P=.002). The genotype frequency of Rs5744174 in TLR5 had a significant association with the presence of H pylori infection (P=.046, OR=0.52). Except for gender (P=.022), there were no significant associations between clinical and demographic variables and SNPs relating to the severity of the H pylori infections.ConclusionsOur findings are consistent with differences in severity of H pylori infection due to TLR SNPs in different ethnic groups. Understanding differences in genetic susceptibility could help in classifying patients and matching patients with various treatment options on a genetic basis.LimitationsLack of H pylori pathogenicity features assessment.Conflicts Of InterestNone.

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