• Stem Cells Transl Med · Jun 2015

    Bioengineering a human plasma-based epidermal substitute with efficient grafting capacity and high content in clonogenic cells.

    • Maia M Alexaline, Marina Trouillas, Muriel Nivet, Emilie Bourreau, Thomas Leclerc, Patrick Duhamel, Michele T Martin, Christelle Doucet, Nicolas O Fortunel, and Jean-Jacques Lataillade.
    • Biomedical Research Institute of French Armies, INSERM U1197, Clamart, France; Celogos, Paris, France; Alternative Energies and Atomic Energy Commission, Institute of Cellular and Molecular Radiobiology, Laboratory of Genomics and Radiobiology of Keratinopoiesis, INSERM UMR 967, Evry, France; Burn Treatment Unit, Percy Hospital, Clamart, France; Plastic Surgery Department, Percy Hospital, Clamart, France.
    • Stem Cells Transl Med. 2015 Jun 1; 4 (6): 643-54.

    UnlabelledCultured epithelial autografts (CEAs) produced from a small, healthy skin biopsy represent a lifesaving surgical technique in cases of full-thickness skin burn covering >50% of total body surface area. CEAs also present numerous drawbacks, among them the use of animal proteins and cells, the high fragility of keratinocyte sheets, and the immaturity of the dermal-epidermal junction, leading to heavy cosmetic and functional sequelae. To overcome these weaknesses, we developed a human plasma-based epidermal substitute (hPBES) for epidermal coverage in cases of massive burn, as an alternative to traditional CEA, and set up critical quality controls for preclinical and clinical studies. In this study, phenotypical analyses in conjunction with functional assays (clonal analysis, long-term culture, or in vivo graft) showed that our new substitute fulfills the biological requirements for epidermal regeneration. hPBES keratinocytes showed high potential for cell proliferation and subsequent differentiation similar to healthy skin compared with a well-known reference material, as ascertained by a combination of quality controls. This work highlights the importance of integrating relevant multiparameter quality controls into the bioengineering of new skin substitutes before they reach clinical development.SignificanceThis work involves the development of a new bioengineered epidermal substitute with pertinent functional quality controls. The novelty of this work is based on this quality approach.©AlphaMed Press.

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