• Int. J. Clin. Pract. · Nov 2021

    Hypercholesterolemic patients have higher eryptosis and erythrocyte adhesion to human endothelium independently of statin therapy.

    • Antonio Cilla, Gabriel López-García, Víctor Collado-Díaz, María Amparo Blanch-Ruiz, Guadalupe Garcia-Llatas, Reyes Barberá, Maria Angeles Martinez-Cuesta, José T Real, Ángeles Álvarez, and Sergio Martínez-Hervás.
    • Nutrition and Food Science Area, Faculty of Pharmacy, University of Valencia, Valencia, Spain.
    • Int. J. Clin. Pract. 2021 Nov 1; 75 (11): e14771.

    BackgroundPhosphatidylserine (PS) externalization out of the membrane facilitates the eryptotic erythrocytes (EE) binding to endothelial cells (EC), potentially leading to atherosclerosis. Thus, the levels of eryptosis and interactions of EE-EC in hypercholesterolemic patients, either non-medicated or medicated, compared with healthy subjects were studied.MethodsA total of 56 subjects clustered into three groups: (control (n = 20), hypercholesterolemic non-treated (HCNT) (n = 15), and statin-treated (HCT) (n = 21)) were enrolled in this cross-sectional study. Biochemical parameters were determined with validated and standard methods. PS exposure was estimated from annexin-V-binding, cell volume from forward scatter (FSC), and GSH from CMFDA fluorescence by flow cytometry. The erythrocyte-EC adhesion assay was performed by using the parallel-plate flow chamber technique.ResultsHigher PS externalization and adhesion of erythrocytes to EC (P < .05) was found in hypercholesterolemic subjects, regardless of statin treatment, compared with the control group. Although no correlation between FSC and PS externalization with other parameters was found, GSH was inversely correlated with erythrocyte adhesion, which was significantly correlated with total cholesterol, LDL-c, and apolipoprotein B.ConclusionThe link between hypercholesterolemia and eryptosis suggests a possible detrimental impact of this binomial on endothelial function with possible further development of atherosclerosis and microcirculation problems in hypercholesterolemic patients, independently of statin therapy.© 2021 The Authors. International Journal of Clinical Practice published by John Wiley & Sons Ltd.

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