• Kazuo Kitagawa, Moeko Saitoh, Kentaro Ishizuka, and Satoru Shimizu.
• Department of Neurology, Tokyo Women's Medical University School of Medicine, Tokyo, Japan. Electronic address: Kitagawa.kazuo@twmu.ac.jp.
• J Stroke Cerebrovasc Dis. 2018 Apr 1; 27 (4): 831-838.

BackgroundRemote ischemic conditioning (RIC) induces protection in focal cerebral ischemia. The conditioning is divided into pre-, per-, and postconditioning. However, the mechanisms of RIC remain unknown.ObjectivesThis study aimed to determine the most effective subtype of RIC. We also examined involvement of collateral circulation on RIC.MethodsTransient middle cerebral artery occlusion (MCAO) was performed with nylon sutures in adult C57BL/6 mice under the monitoring of cerebral blood flow (CBF). Fifty mice were divided into 5 groups: MCAO control group, delayed pre-RIC group (RIC 24 hours before MCAO), early pre-RIC group (RIC 5 minutes before MCAO), per-RIC group (RIC during MCAO), and post-RIC group (RIC 5 minutes after MCAO). In other middle cerebral artery (MCA) control and per-RIC groups, collateral circulation was visualized with latex compound perfusion.ResultsAfter MCAO, CBF was reduced by 80% in all groups. At the end of MCAO, relative increase in CBF in per-RIC group was significantly greater than that in MCA control, whereas the infarct volume in per-RIC group was significantly smaller than that in other groups. The diameter of leptomeningeal anastomosis was larger in the per-RIC group than that in the control group.ConclusionsAmong the 4 RIC procedures, only the per-RIC group showed clear brain protection. Enhancement of collateral circulation could play a role in the protective effect of per-RIC.Copyright © 2018 National Stroke Association. Published by Elsevier Inc. All rights reserved.

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