• Ariadne Stavare Leal Araújo, Carina Verna, Osvaldo Pereira Araújo Júnior, José Maria Soares Júnior, Ricardo Santos Simões, Edmund Chada Baracat, SimõesManuel de JesusMJhttp://orcid.org/0000-0003-2770-8618Universidade Federal de São Paulo, Morphology and Genetics Department - São Paulo (SP), Brazil., and GomesRegina Célia TeixeiraRCThttp://orcid.org/0000-0001-6657-1920Universidade Federal de São Paulo, Morphology and Genetics Department - São Paulo (SP), Brazil.Universidade de São Paulo, Faculty of Medicine Obstetrics and Gynecology Department - São Paulo.
• Universidade Federal de São Paulo, Morphology and Genetics Department - São Paulo (SP), Brazil.
• Rev Assoc Med Bras (1992). 2021 Jun 1; 67 (5): 650-655.

ObjectiveTo assess the impact of the metoclopramide-induced hyperprolactinemia in cellular death and proliferation in the harderian gland of female mice.MethodsTwenty female mice were divided into two groups of 10 animals each and treated: 0.2 mL of saline solution (controls, Ctr) and 200 µg of metoclopramide (experimental, hyperprolactinemia), both for 50 consecutive days and at 12:00 a.m. On the 50th day, the female were euthanized, and the harderian glands were removed and processed for immunohistochemistry for detected ki67 and TUNEL method. Data were statistically analyzed by unpaired Student's t test (p<0.05).ResultsThe harderian gland of the hyperprolactinemia group showed increase in the immunoexpression of Ki67 and TUNEL compared to the Ctr group (p<0.05), and there was no significant difference in the amount of porphyrin in the HPrl group compared to the Ctr group.ConclusionThe hyperprolactinemia led to increased cell death in the acini the harderian gland and cell proliferation in the stroma glandular, fact that suggesting a reduction process of cellular activity and fibrosis, which suggests impairment in the functioning of the lacrimal harderian.

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