• Medicina · Sep 2021

    Production of a Soluble Recombinant Antibody Fragment against MMP9 Using Escherichia coli.

    • Chang-Hun Yeom and Hee-Jin Jeong.
    • Department of Biological and Chemical Engineering, Hongik University, Sejong 30016, Korea.
    • Medicina (Kaunas). 2021 Sep 18; 57 (9).

    AbstractMatrix metalloproteinase 9 (MMP9) is involved in several aspects of the pathology of cancer, including invasion, metastasis, and angiogenesis. In this study, we expressed a recombinant scFv-type anti-MMP9 antibody in soluble form using Escherichia coli, purified it, and confirmed its antigen-binding ability. The convenient, rapid, inexpressive system used in this study for producing recombinant antibody fragments needs only five days, and thus can be used for the efficient production of scFv against MMP9, which can be used in a range of applications and industrial fields, including diagnosis and treatment of inflammatory and cancer-related diseases.

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