• Amel Hassan, Pamela Lee, Paraskevi Maggina, Jin Hua Xu, Diana Moreira, Mary Slatter, Zohreh Nademi, Austen Worth, Stuart Adams, Alison Jones, Catherine Cale, Zoe Allwood, Kanchan Rao, Robert Chiesa, Persis Amrolia, Hubert Gaspar, E Graham Davies, Paul Veys, Andrew Gennery, and Waseem Qasim.
• Immunology and Bone Marrow Transplant Units, Great Ormond Street Hospital for Children, London, United Kingdom.
• J. Allergy Clin. Immunol. 2014 Jun 1; 133 (6): 1660-6.

BackgroundSevere combined immunodeficiency (SCID) can be cured by using allogeneic hematopoietic stem cell transplantation, and the absence of host immunity often obviates the need for preconditioning. Depending on the underlying genetic defect and when blocks in differentiation occur during lymphocyte ontogeny, infants with SCID have absent or greatly reduced numbers of functional T cells. Natural killer (NK) cell populations are usually absent in the SCID-X1 and Janus kinase 3 forms of SCID and greatly reduced in adenosine deaminase deficiency SCID but often present in other forms of the disorder.ObjectiveTo determine if SCID phenotypes indicate host permissiveness to donor cell engraftment.MethodsA retrospective data analysis considered whether host NK cells influenced donor T-cell engraftment, immune reconstitution, and long-term outcomes in children who had undergone nonconditioned allogeneic stem cell transplantation between 1990 and 2011 in the United Kingdom. Detailed analysis of T- and B-cell immune reconstitution and donor chimerism was compared between the NK(+) (n = 24) and NK(-) (n = 53) forms of SCID.ResultsOverall, 77 children underwent transplantation, with survival of 90% in matched sibling donor/matched family donor transplants compared with 60% when alternative donors were used. Infants with NK(-)SCID were more likely to survive than NK(+) recipients (87% vs 62%, P < .01) and had high-level donor T-cell chimerism with superior long-term recovery of CD4 T-cell immunity. Notably, 33% of children with NK(+)SCID required additional transplantation procedures compared with only 8% of children with NK(-)SCID (P < .005).ConclusionsNK(-)SCID disorders are highly permissive for donor T-cell engraftment without preconditioning, whereas the presence of NK cells is a strong indicator that preparative conditioning is required for engraftment of T-cell precursors capable of supporting robust T-cell reconstitution.Copyright © 2014 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.

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