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Nan Fang Yi Ke Da Xue Xue Bao · Aug 2015
[Minocycline reduces hyperpolarization-activated current in rat substantia gelatinosa neurons].
- Mengye Zhu, Nana Liu, Sicong Peng, Lingchao Li, Daying Zhang, and Tao Liu.
- Department of Pain Clinic, First Affiliated Hospital of Nanchang University, Nanchang 330006, China. E-mail: zhumengye0403@163.com.
- Nan Fang Yi Ke Da Xue Xue Bao. 2015 Aug 1; 35 (8): 1155-61.
ObjectiveTo investigate the effect of minocycline on hyperpolarization-activated current (Ih) in the substantia gelatinosa (SG) neurons in rat spinal dorsal horn.MethodsIn vitro spinal cord transverse slices were prepared from 3-5-week-old male Sprague-Dawley rats. Using whole-cell patch clamp technique, Ih currents were recorded before and after bath application of minocycline (1-300 µmol/L) to the SG neurons.ResultsIh currents were observed in nearly 50% of the recorded neurons, and were blocked by Ih blocker CsCl and ZD7288. Minocycline rapidly and reversibly reduced the amplitude of Ih and decreased the current density in a concentration-dependent manner with an IC50 of 34 µmol/L.ConclusionMinocycline suppresses the excitability of SG neurons through inhibiting the amplitude and current density of Ih and thereby contributes to pain modulation.
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