• J Res Med Sci · Nov 2013

    Differential proteomics analysis of proteins from human diabetic and age-related cataractous lenses.

    • Jing Zhu, Jun Shao, Yong Yao, Zhao Dong Chu, Qian Qian Yu, Wei Zhao, Qing Lin, and Zi Yin Zhang.
    • Department of Ophthalmology, Nanjing Medical University Affiliated Wuxi People's Hospital, Wuxi, Jiangsu, People's Republic of China.
    • J Res Med Sci. 2013 Nov 1; 18 (11): 984-9.

    BackgroundTo investigate the differential lens proteomics between diabetic cataract, age-related cataract, and natural subjects.Materials And MethodsTwo-dimensional electrophoresis (2-DE), mass spectrometry (MS), and enzyme-linked immunosorbent assay (ELISA) were employed. Total soluble proteins in lenses of type I diabetic cataract, age-related cataract (nondiabetic) patients, and normal control were extracted and subjected to 2-DE. The differential protein spots were recovered, digested with trypsin, and further applied to MALDI-TOF-MS. ELISA analysis was used to determine the levels of differential proteins in lenses of three groups.Results2-DE analysis reflected that lens proteins of normal control, diabetic, and age-related cataract subjects were in the section of pH 5-9 and the relative molecular weights were 14-97 kDa, while relative molecular weight of more abundant crystallines was localized at 20-31 kDa. five differential protein spots were detected and identified using MALDI-TOF-MS, including beta-crystallin A3, alpha-crystallin B chain, chain A of crystal structure of truncated human beta-B1-crystallin, beta-crystallin B1, and an interesting unnamed protein product highly similar to alpha-crystallin B chain, respectively. ELISA analysis revealed that lenses of diabetic cataract patients should contain significantly more concentrations of beta-crystallin A3, alpha-crystallin B chain, and beta-crystallin B1 than those of age-related cataract patients and normal control.ConclusionThis study clearly reflected the differential proteins of diabetic cataract, age-related cataract lenses compared with natural subjects, and it is helpful for the further research on the principles and mechanisms of different types of cataract.

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