• Behrouz Keleidari, Mohsen Mahmoudieh, Faranak Bahrami, Pejman Mortazavi, Reza Sari Aslani, and Seyed Alireza Toliyat.
• Department of Surgery, Faculty Member of General Surgery, Isfahan University of Medical Sciences, Isfahan, Iran.
• J Res Med Sci. 2014 Jan 1; 19 (1): 28-32.

BackgroundThe aim of this study is to investigate the effect of vitamin A and C, as the agents that improve wound healing, on the adhesion formation process.Materials And MethodsSixty male Wistar rats were used. They underwent midline laparotomy, for repair of a peritoneal injury, and were then assigned to four groups. Group 1 (Vitamin A) received 2000 units/kg intramuscular injection of vitamin A daily, post surgery, for two weeks; Group 2 (Vitamin C) received 100 mg/kg oral vitamin C daily, after laparotomy, for two weeks; Group 3 (vitamins A and C) received 2000 units/kg intramuscular injection of vitamin A and 100 mg/kg oral vitamin C daily, after laparotomy, for two weeks, and Group four (Sham) rats did not receive any drugs. The adhesion, inflammation, fibrosis scores, and wound integrity were evaluated after two weeks.ResultsRats in the vitamin C group had the lowest mean adhesion formation score (1 ± 0.27) and the values of p were < 0.0001 for the vitamin A group and vitamin A and C groups and 0.003 for the sham group. Vitamin C also had the lowest fibrosis score (0.50 ± 0.17) among the study groups and the values of p were < 0.0001 for the vitamin A group and vitamin A and C groups and 0.002 for the sham group. The mean inflammation score did not differ significantly among the study groups. The wound disruption strength was the highest in the vitamin C group and the difference was statistically significant in the sham group (1188.69 ± 281.92 vs. 893.04 ± 187.46, p : 0.003).ConclusionAdministration of oral vitamin C reduces adhesion formation and improves wound healing.

3 keywords...

hide…