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- Bahareh Vakili, Farzin Khorvash, Hossein Fazeli, and Moj Khaleghi.
- Department of Microbiology, Science and Research Branch, Islami Azad University, Kerman, Iran.
- J Res Med Sci. 2014 Jun 1; 19 (6): 567-70.
BackgroundThe purpose of this study was to screen of parC gene mutations in clinical isolates of Acinetobacter baumannii from intensive care units (ICUs) of Alzahra Hospital, Isfahan, Iran.Materials And MethodsSeventy isolates of A. baumannii between March 2011 and June 2012 were studied. Susceptibility test was established by E-test method. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing was performed for detection of parC gene mutation.Results77.1% of specimens were highly resistant. Mutation at position 80 in parC was observed in 93% of isolates.ConclusionHigh proportion of A. baumannii isolates had a mutation in parC that can play an important role in increased incidence of these isolates.
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