• Ivana Okić-Djordjević, Drenka Trivanović, Miloš Jovanović, Marija Ignjatović, Bojana Šećerov, Miloš Mojović, Diana Bugarski, Goran Bačić, and Pavle R Andjus.
• Pavle R. Andjus, Faculty of Biology University of Belgrade, Studentski trg 12, 11000 Belgrade, Serbia, pandjus@bio.bg.ac.rs.
• Croat. Med. J. 2014 Feb 1; 55 (1): 45-9.

AimTo investigate the survival of laboratory rats after irradiation and to study the cellularity of their bone marrow and the multipotential mesenchymal stem cells (BM-MSCs) in groups treated with or without a new thiol-based radioprotector (GM2011).MethodsAnimals were irradiated by a Cobalt gamma source at 6.7 Gy. Treated animals were given i.p. GM2011 30 minutes before and 3 and 7 hours after irradiation. Controls consisted of sham irradiated animals without treatment and animals treated without irradiation. After 30 days post-irradiation, animals were sacrificed and bone marrow cells were prepared from isolated femurs. A colony forming unit-fibroblast (CFU-F) assay was performed to obtain the number of BM-MSCs.ResultsIn the treated group, 87% of animals survived, compared to only 30% in the non-treated irradiated group. Irradiation induced significant changes in the bone marrow of the treated rats (total bone marrow cellularity was reduced by~60%--from 63 to 28 cells × 10(6)/femur and the frequency of the CFU-F per femur by~70% - from 357 to 97), however GL2011 almost completely prevented the suppressive effect observed on day 30 post-irradiation (71 cells × 10(6)/femur and 230 CFU-F/femur).ConclusionAlthough the irradiation dosage was relatively high, GL2011 acted as a very effective new radioprotector. The recovery of the BN-MSCs and their counts support the effectiveness of the studied radioprotector.

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