• Diagn. Microbiol. Infect. Dis. · Feb 2021

    Sample pooling on the Cepheid Xpert® Xpress SARS-CoV-2 assay.

    • Maryza Graham, Eloise Williams, Nicole Isles, Eka Buadromo, Tebuka Toatu, Julian Druce, Mike Catton, Chantel Lin, Benjamin P Howden, and Deborah A Williamson.
    • Microbiological Diagnostic Unit, Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Australia; Department of Microbiology, Monash Health, Melbourne, Australia. Electronic address: Maryza.Graham@monashhealth.org.
    • Diagn. Microbiol. Infect. Dis. 2021 Feb 1; 99 (2): 115238.

    AbstractThe COVID-19 pandemic has placed unprecedented global demand on laboratory supplies required for testing. Sample pooling has been investigated by laboratories as a strategy to preserve testing capacity. We evaluate the performance of Cepheid Xpert® Xpress SARS-CoV-2 RT-PCR assay for testing samples in pools of 4 and 6. Clinical samples containing SARS-CoV-2, and confirmed negative clinical samples were used to create sample pools. Clinical samples had 'neat' Xpert® E gene cycle threshold values ranging between 20 and 28 and all were detected qualitatively when contained in pools of 4 or 6 samples. For these samples, pooling had a median change in cycle threshold value of 2.0 in pools of 4, and of 2.9 in pools of 6. With the use of Cepheid Xpert® Xpress SARS-CoV-2 RT-PCR assay, pooling of 4 or 6 samples may be an effective strategy to increase testing capacity.Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.

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