• Ann Trop Paediatr · Dec 1983

    Use of buffy-coat smears in the diagnosis of septicaemia in children.

    • F K Nkrumah, J Neequaye, and K Twum-Danso.
    • Ann Trop Paediatr. 1983 Dec 1; 3 (4): 193-6.

    AbstractGram-stained buffy-coat smears from venous and capillary blood samples in 105 children suspected of septicaemia were examined for the presence of bacteria and the results compared with blood culture isolates. Gram-positive and Gram-negative bacteria were identified in 18 venous (44%) and 19 capillary (46%) buffy-coat preparations in 41 instances where bacterial organisms were isolated from the blood cultures. It is concluded that the examination of buffy-coat smears for bacteria in children suspected of septicaemia is a useful adjunct to blood cultures and, in areas where no facilities exist for culture of blood, may be a simple and rapid method of establishing the diagnosis of bacteraemia in suspected patients.

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