• Yale J Biol Med · Sep 1983

    New laboratory techniques for isolation of Mycoplasma pneumoniae.

    • J G Tully.
    • Yale J Biol Med. 1983 Sep 1; 56 (5-6): 511-5.

    AbstractThe SP-4 culture medium, developed originally for newly isolated plant and insect mycoplasmas (spiroplasmas), has markedly improved the recovery of Mycoplasma pneumoniae from human clinical materials. This medium, in combination with a direct fluorescent antibody test, can enhance the recovery and identification of the organism by 30-40 percent over conventional culture procedures. Although these modifications are a clear improvement in diagnostic techniques for M. pneumoniae, the time required for growth and identification of the agent is still a major disadvantage for rapid clinical diagnosis. Thus, there remains a critical need for techniques that can specifically identify the major antigens (or other components) of the organism within the first week of the infection.

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