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Clin. Microbiol. Infect. · Feb 2011
Comparative StudyThe sensitivity of direct identification from positive BacT/ALERT™ (bioMérieux) blood culture bottles by matrix-assisted laser desorption ionization time-of-flight mass spectrometry is low.
- F Szabados, M Michels, M Kaase, and S Gatermann.
- Institute for Hygiene and Microbiology, Department of Medical Microbiology, Ruhr-University Bochum, Bochum, Germany. florian.szabados@rub.de
- Clin. Microbiol. Infect. 2011 Feb 1; 17 (2): 192-5.
AbstractRecently, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been presented as a novel method for the direct identification of bacteria from positive blood culture bottles. The rate of the MALDI TOF MS-based identification in the present study from positive BacT/ALERT (bioMérieux, Marcy l'Etoile, France) blood culture bottles was 30%, which is far below the previously reported sensitivities using the BACTEC (Becton Dickinson, Franklin Lakes, NJ, USA) system. We also found evidence that the Biotyper algorithm did not identify a second pathogen in cases of positive BacT/ALERT blood culture bottles containing two different species.© 2010 The Authors. Journal Compilation © 2010 European Society of Clinical Microbiology and Infectious Diseases.
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