• Savannah Smith, Jessica Heffner, Rachael King, and Haley Guzman.
• 1st Armored Brigade Combat Team, 1st Infantry Division, Fort Riley, KS 66442, USA.
• Mil Med. 2023 May 16; 188 (5-6): e957e962e957-e962.

IntroductionThis brief report describes the process, used by the 1st Infantry Division (1ID) and Irwin Army Community Hospital (IACH) at Fort Riley, Kansas, for conducting pooled testing collection of over 2,500 Soldiers prior to a large-scale exercise involving multiple units.Materials And MethodsThe authors captured after action review comments on the process and results of their pooled specimen collection site. Pooled specimen test results were reviewed and classified according to Aberdeen Proving Ground criteria to determine the percentage of successful and failed pooled specimens.Results1ID and IACH performed pooled testing collection and shipment of 2,684 specimens divided into 298 pools over 6 flight manifests. Of the 298 pooled specimens, 4 (1.34%) were found to be inconclusive or invalid, and the other 294 (98.7%) had sufficient number of human cells to be certified as SARS-CoV-2 (COVID-19) positive or COVID-19 not detected.ConclusionPooled testing collection is a complex process that may continue to be a requirement for mass screening of COVID-19 prior to military operations. While planning should be tailored to the specific mission and unit, key factors that the authors feel are required for pooled testing to be successful in any situation are standardized training and personnel continuity, quality assurance, administrative oversight by the unit, and collaboration and communication between all involved entities.Published by Oxford University Press on behalf of the Association of Military Surgeons of the United States 2021. This work is written by (a) US Government employee(s) and is in the public domain in the US.

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