• Molecules · Sep 2020

    Review

    Mass Spectrometry and Structural Biology Techniques in the Studies on the Coronavirus-Receptor Interaction.

    • Danuta Witkowska.
    • Institute of Health Sciences, Opole University, Katowicka 68, 45-060 Opole, Poland.
    • Molecules. 2020 Sep 10; 25 (18).

    AbstractMass spectrometry and some other biophysical methods, have made substantial contributions to the studies on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and human proteins interactions. The most interesting feature of SARS-CoV-2 seems to be the structure of its spike (S) protein and its interaction with the human cell receptor. Mass spectrometry of spike S protein revealed how the glycoforms are distributed across the S protein surface. X-ray crystallography and cryo-electron microscopy made huge impact on the studies on the S protein and ACE2 receptor protein interaction, by elucidating the three-dimensional structures of these proteins and their conformational changes. The findings of the most recent studies in the scope of SARS-CoV-2-Human protein-protein interactions are described here.

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