• Xuemei Wang, Shun Yu, Fangfei Li, and Tao Feng.
• Center for Neurodegenerative Disease, Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, #6 Tian Tan Xi Li Street, 100050 Beijing, China. Electronic address: minnie02@sina.com.
• Neurosci. Lett. 2015 Jul 10; 599: 115-9.

AbstractParkinson's disease (PD) is a neurodegenerative disorder characterized by intracellular α-synuclein (α-syn) deposition. Alterations in α-syn levels in cerebrospinal fluid (CSF) and plasma of PD patients have been thought to be potential PD biomarkers; however, contamination arising from hemolysis often influences the accuracy of detecting α-syn levels in the CSF and plasma. In this study, α-syn oligomer levels in red blood cells (RBCs) obtained from 100 PD patients, 22 MSA patients, and 102 control subjects were measured by enzyme-linked immunosorbent assay. We showed that the ratio of α-syn oligomer/total RBC protein was higher in PD patients than in controls (29.0±19.8 ng/mg vs. 15.4±7.4 ng/mg, P<0.001). The area under the receiver operating characteristic curve (AUC) indicated a sensitivity of 79.0%, specificity of 64.7% and a positive predictive value of 68.7%, with an AUC of 0.76 for increased α-syn oligomer/total RBC protein ratio. However, there was no correlation between RBC α-syn oligomer levels and age at onset, disease duration, age, UPDRS motor scale score or progression of motor degeneration in PD patients. The ratio of RBC α-syn oligomer/total protein was also higher in MSA patients than in controls (22.9±13.9 ng/mg vs. 15.4±7.4 ng/mg, P<0.001). However, no significant difference was found for α-syn oligomer/total protein ratio between PD and MSA (29.0±19.8 ng/mg vs. 22.9±13.9 ng/mg, P>0.05). The present results suggest that the RBC α-syn oligomer/total protein ratio can be a potential diagnostic biomarker for PD.Copyright © 2015. Published by Elsevier Ireland Ltd.

20 keywords...

hide…