• Croatian medical journal · Dec 2019

    Expression and localization of DAB1 and Reelin during normal human kidney development.

    • Anita Racetin, Marija Jurić, Natalija Filipović, Ivana Šolić, Ivona Kosović, Merica Glavina Durdov, Nenad Kunac, Sandra Zekić Tomaš, Marijan Saraga, Violeta Šoljić, Vlatka Martinović, Joško Petričević, Ivana Restović, Valentina Lasić, Sandra Kostić, Boris Kablar, Koichiro Watanabe, Yu Katsuyama, Saraga BabićMirnaM, and Katarina Vukojević.
    • Katarina Vukojevic, Department of Anatomy, Histology and Embryology, Laboratory for Early Human Development, University of Split, School of Medicine, Šoltanska 2, 21000 Split, Croatia, katarina.vukojevic@mefst.hr.
    • Croat. Med. J. 2019 Dec 31; 60 (6): 521-531.

    AimTo explore the spatial and temporal expression patterns of DAB1 and Reelin in the developing and postnatal healthy human kidneys as potential determinants of kidney development.MethodsParaffin-embedded fetal kidney tissue between the 13/14th and 38th developmental weeks (dw) and postnatal tissue at 1.5 and 7 years were stained with DAB1 and Reelin antibodies by double immunofluorescence.ResultsDuring the fetal kidney development and postnatal period, DAB1 and Reelin showed specific spatial expression pattern and diverse fluorescence intensity. During the fetal period, DAB1 was strongly expressed in the distal convoluted tubules (DCT), with strong reactivity, and diversely in the proximal convoluted tubules (PCT) and glomeruli. In the postnatal period, DAB1 expression decreased. The strongest Reelin expression in early fetal stages was observed in the PCT. In the postnatal period, Reelin expression decreased dramatically in all observed structures. These two markers were colocalized during early developmental stages, mostly in PCT, DCT, and podocytes.ConclusionThe appearance of DAB1 and Reelin during fetal kidney development confirms their potential significant role in the formation of kidney structure or function. High DAB1 expression in the DCT implies its regulatory role in tubular formation or function maintenance during development. Reelin was highly expressed in human kidneys at early fetal stages, mostly in the PCT, while at later fetal stages and postnatal period its expression decreased.

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