• Clinics · Jan 2022

    MicroRNA-128b mediates lipopolysaccharide-induced apoptosis via reactive oxygen species in human pulmonary microvascular endothelial cells.

    • Guangwen Long, Xiulin Yang, Chunling Ji, and Yukang Dong.
    • Emergency Department of Internal Medicine, Guizhou Provincial People's Hospital, Guiyang, China. Electronic address: longguangwen2006@163.com.
    • Clinics (Sao Paulo). 2022 Jan 1; 77: 100020.

    ObjectivesThis study aimed to explore the effects of miR-128b in the regulation of Lipopolysaccharide (LPS) induced apoptosis.MethodsHuman Pulmonary Microvascular Endothelial Cells (HPMECs) were transfected with an miR-128b inhibitor and stimulated with LPS for 24 h. FCM was performed to detect apoptosis and Reactive Oxygen Species (ROS) production. In addition, miRNA and caspase-3 expression levels were determined using real-time quantitative polymerase chain reaction and western blotting.ResultsLPS significantly induced apoptosis and ROS production and upregulated miR-128b and caspase-3 expressions in HPMECs. However, LPS-induced effects were suppressed when an miR-128b inhibitor was used. Preincubation with NAC decreased the LPS-induced apoptosis of HPMECs.ConclusionsThese effects were mediated by miR-128b via the caspase-3 pathway.Copyright © 2022 HCFMUSP. Published by Elsevier España, S.L.U. All rights reserved.

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