• Croatian medical journal · Jun 2022

    Medicinal signaling cells niche in stromal vascular fraction from lipoaspirate and microfragmented counterpart.

    • Lucija Zenić, Denis Polančec, Damir Hudetz, Zeljko Jeleč, Eduard Rod, Dinko Vidović, Mario Starešinić, Srećko Sabalić, Trpimir Vrdoljak, Tadija Petrović, Fabijan Čukelj, Vilim Molnar, Martin Čemerin, Vid Matišić, Petar Brlek, Zrinka Djukić Koroljević, Igor Borić, Gordan Lauc, and Dragan Primorac.
    • Lucija Zenić, Srebrnjak Children's Hospital, Srebrnjak 100, 10000 Zagreb, Croatia, lzenic@bolnica-srebrnjak.hr.
    • Croat. Med. J. 2022 Jun 22; 63 (3): 265-272.

    AimTo expand our previous findings by increasing the number of patients in a study characterizing medicinal signaling cells (MSC) of stromal vascular fraction from lipoaspirate (SVF-LA) and from microfragmented lipoaspirate (SVF-MLA) applied for the treatment of osteoarthritis (OA).MethodsTwenty OA patients, including 8 new patients, acquiring autologous microfragmented adipose tissue were enrolled. In-parallel immunophenotyping of SVF-LA and SVF-MLA was performed. The samples were incubated in a DuraClone SC prototype tube targeting the CD31, CD34, CD45, CD73, CD90, CD105, and CD146 surface markers, stained with the DRAQ7 cell nuclear dye and Live/Dead Yellow Fixable Stain, and analyzed by flow cytometry.ResultsThe population phenotypes in SVF-LA and SVF-MLA samples included CD31+CD34+CD73±CD90±CD105±CD146± endothelial progenitors (EP), CD31+CD34-CD73±CD90±CD105-CD146± mature endothelial cells, CD31-CD34-CD73±CD90+CD105-CD146+ pericytes, CD31-CD34+CD73±CD90+CD105-CD146+ transitional pericytes, and CD31-CD34+CD73highCD90+CD105-CD146- supra-adventitial-adipose stromal cells. Compared with the autologous SVF-LA samples, the prevailing cell type in SVF-MLA were EP, which outnumbered leukocytes and supra-adventitial-adipose stromal cells (SA-ASC). The ratio of progenitor cells in SVF-MLA samples differed between female and male patients, showing a higher EP-pericyte and pericyte-SA-ASC ratio in men.ConclusionOur results, hallmarked by EP-enriched anti-inflammatory features and indicating a possible sex-specific impact, contribute to defining the cellular composition of the clinically applied MSC serving as a regenerative cell therapy in OA.

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