• Yang Pan, Dan Wang, and Fan Liu.
• Department of Laboratory Medicine, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, China.
• Clinics (Sao Paulo). 2022 Jan 1; 77: 100069.

ObjectivesM1 macrophage polarization and phenotype in Inflammatory Bowel Disease (IBD) are common biological responses.MethodHerein, IBD mice models were constructed and macrophages were derived.ResultsIt was discovered that microRNA-146b (miR-146b) was downregulated in IBD mice and Lipopolysaccharide (LPS)-induced macrophages. Moreover, the inhibitory role of overexpressed miR-146b in reducing the inflammation level and blocking M1 macrophage polarization was confirmed. Further investigation indicated that Fibrinogen Like 2 (FGL2) acted as the target gene of miR-146b, and FGL2 mediated activation of NLRP3, NF-κB-p65, and p38-MAPK. More importantly, it was validated that miR-146b could ameliorate inflammatory phenotype and prevent M1 macrophage polarization via inhibiting FGL2 in vitro, and miR-146b overexpression alleviated the intestinal injury of IBD mice in vivo.ConclusionsOverall, it is potential to use miR-146b for the amelioration of IBD.Copyright © 2022 HCFMUSP. Published by Elsevier España, S.L.U. All rights reserved.

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