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- Márcia Carolina Mazzaro, Émelin Alves Dos Santos, Gessica Baptista de Melo, Priscila Duarte Marques, Laura Vilela Souza, Jefferson Elias-Oliveira, Bruna Campos da Silva, Ronaldo César Borges Gryschek, Fabiana Martins de Paula, and Rosângela Maria Rodrigues.
- Laboratório de Parasitologia, Universidade Federal de Jataí, Jataí, GO, Brazil.
- Clinics (Sao Paulo). 2022 Jan 1; 77: 100060.
ObjectiveThe association between diabetes and Strongyloides infection remains controversial. This study aimed to detect Strongyloides stercoralis DNA in the feces of patients with Diabetes Mellitus type 2 (DM2).MethodsFecal samples were analyzed via the Lutz, Rugai, and agar plate culture methods. PCR amplification was performed using two targets (PCR-genus and PCR-species) located on the S. stercoralis 18S ribosomal.ResultsThe positivity for S. stercoralis using parasitological methods was 1.1%. PCR-genus (14.13%) demonstrated a higher positivity than PCR-species (9.78%).ConclusionThe results confirm the greater positivity of the molecular diagnosis in relation to parasitological methods, reinforcing its use as an additional tool for the diagnosis of S. stercoralis infection in patients with DM2 living in endemic areas for this helminthiasis.Published by Elsevier España, S.L.U.
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