• Clinics · Jan 2022

    TRIM47 promotes ovarian cancer cell proliferation, migration, and invasion by activating STAT3 signaling.

    • Xi Wang, Yu Fu, and Yanyan Xing.
    • Department of Emergency and Critical Care Medicine, Jinshan Hospital, Fudan University, Shanghai, China.
    • Clinics (Sao Paulo). 2022 Jan 1; 77: 100122100122.

    ObjectivesTripartite Motif 47 (TRIM47) protein plays a prominent role in many cancers. This study aimed to investigate the biological roles of TRIM47 in ovarian cancer.MethodsTRIM47 was knocked down and overexpressed in ovarian cancer cell lines SKOV3 and OVCAR3, and the effects on proliferation, clone formation, apoptosis, invasion, and growth of xenograft tumors in nude mice were determined. The expression levels of the selected candidates were tested by western blotting and quantitative real-time PCR.ResultsTRIM47 knockdown suppressed proliferation and encourages apoptosis of ovarian cancer cells. Similarly, TRIM47 knockdown suppressed ovarian cancer cell invasion, migration, and epithelial-mesenchymal transition. Ovarian cancer cell xenograft assays demonstrated that TRIM47 knockdown significantly inhibited tumor growth. Mechanistically, TRIM47 knockdown suppressed STAT3 phosphorylation and the expression of several downstream genes, including MCL-1, MMP2, and c-MYC. Silencing of STAT3 partially prevented TRIM47-induced tumor cell proliferation and invasion.ConclusionThe present study's findings demonstrate that by activating STAT3 signaling, TRIM47 functions as an oncogene in ovarian cancer. TRIM47, therefore, appears to be a potential target for ovarian cancer prevention and/or therapy.Copyright © 2022 HCFMUSP. Published by Elsevier España, S.L.U. All rights reserved.

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