• Jaime López M, Carmen Gloria Ili G, Priscilla Brebi M, Patricia García M, Italo Capurro V, Pablo Guzmán G, Eugenio Suárez P, José Manuel Ojeda F, and Juan Carlos Roa S.
• Laboratorio de Patología Molecular, Departamento de Anatomía Patológica, Facultad de Medicina/Núcleo de Desarrollo Científico Tecnológico en Biorecursos, Universidad de la Frontera, Temuco, Chile.
• Rev Med Chil. 2010 Nov 1; 138 (11): 134313501343-50.

BackgroundThe relationship between human papillomavirus (HPV) and uterine cervical cancer (UCC) is widely known and accepted.AimTo determine the frequency of genotypes of HPV in cervical preneoplastic lesions in a high risk area of UCC.Material And MethodsUsing a combination of PCR and Reverse Line Blot technique, 235 formalin fixed paraffin embedded samples, with diagnosis of low-grade squamous intraepithelial lesion (LSIL) or high-grade squamous intraepithelial lesion (HSIL) were genotyped.ResultsHPV was detected in 61.2% of LSIL and 78.1% of HSIL. The main genotypes found were HPV 16, 18, 31, 45, 56 y 58. HPV 16 was the most common in both LSIL (18.1%) and HSIL (36.9%). HPV 16 or 18 were present in 25.1% and 47.1% of the LSIL and HSIL respectively. In both LSIL and HSIL, the predominant viral genotypes were those types classified as with a high oncogenic risk.ConclusionsHPV genotypes 16, 18, 31, 45, 56 y 58 were the most common in our series. HPV 16 and 18, viral types with high oncogenic risk and included in commercial vaccines, were found in 25.1% and 47.1% of LSIL and HSIL, respectively.

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