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Chinese Med J Peking · Oct 2003
Unique GGT --> GTT mutation at K-ras codon 12 in six human pancreatic cancer cell lines from Chinese patients.
- Shuanzeng Wei, Tonghua Liu, Hongrui Liu, and Jie Gao.
- Department of Pathology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Science, Beijing, China.
- Chinese Med J Peking. 2003 Oct 1; 116 (10): 158515871585-7.
ObjectiveTo investigate the K-ras mutation pattern in six pancreatic cancer cell lines from Chinese patients.MethodsAll six cell lines were analyzed for mutation in exon 1 of the K-ras gene by polymerase chain reaction (PCR) and direct sequencing.ResultsAll 6 pancreatic cancer cell lines had GGT --> GTT mutation at K-ras codon 12 but no mutations at codon 13.ConclusionThe unique GGT --> GTT mutation at codon 12 plays a potential role in the carcinogenesis of pancreatic cancers in Chinese.
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