• Chung-Yu Huang, Wen-Shin Chang, Chia-Wen Tsai, Te-Chun Hsia, Te-Chun Shen, Da-Tian Bau, and Hao-Ai Shui.
• Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei Taoyuan Armed Forces General Hospital, Taoyuan Terry Fox Cancer Research Laboratory, Translational Medicine Research Center, China Medical University Hospital Graduate Institute of Biomedical Sciences, China Medical University Department of Bioinformatics and Medical Engineering, Asia University, Taichung, Taiwan, R.O.C.
• Medicine (Baltimore). 2018 Sep 1; 97 (36): e12135e12135.

AbstractThe incidence rate of nasopharyngeal cancer (nasopharyngeal carcinoma [NPC]) is much higher in Southeast Asia than in western countries. Interleukin-8 (IL-8), a chemokine produced by macrophages, epithelial cells, airway smooth muscle cells, and endothelial cells, is an important immuno-mediator in the development and progression of many types of cancer. Genetic variations in IL-8 have been associated with the risks of NPC and other cancers. In the current study, we evaluated the role of IL-8 in NPC at the levels of DNA, RNA, and protein in a Taiwanese population. First, in a case-control study, 176 NPC patients and 352 cancer-free controls were genotyped, and the associations of IL-8 T - 251A, C + 781T, C + 1633T, and A + 2767T polymorphisms with NPC risk were evaluated. Second, the NPC tissue samples were assessed for their IL-8 mRNA and protein expression by real-time quantitative reverse transcription polymerase chain reaction (PCR) and Western blotting, respectively. Regarding the IL-8 promoter T - 251A, the TA and AA genotypes were associated with significantly decreased risks of NPC compared with the wild-type TT genotype (adjusted odds ratio = 0.61 and 0.52, 95% confidence interval = 0.47-0.93 and 0.37-0.91, P = .0415 and .0289, respectively). The mRNA and protein expression levels for NPC tissues revealed no significant associations among the 20 NPC samples with different genotypes. These findings suggest that IL-8 may play an important role in the carcinogenesis of NPC in Taiwan.

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