• Larsson Alexander P, Rakar Jonathan, Kratz Gunnar, and Junker Johan P E.
• Laboratory for Experimental Plastic Surgery, Department of Biomedical and Clinical Sciences, Linköping University, SE-581 85, Linköping, Sweden; Department of Hand Surgery, Plastic Surgery, and Burns, Linköping University Hospital, SE-581 85, Linköping, Sweden; Department of Reconstructive Plastic Surgery, Karolinska University Hospital, SE-171 76, Stockholm, Sweden. Electronic address: palexanderlarsson@gmail.com.
• Burns. 2023 Sep 1; 49 (6): 136313711363-1371.

IntroductionRemoval of necrotic tissue is a vital step in the treatment of full-thickness burn wounds, with surgical debridement being the most effective method. Since minor burn wounds are typically treated on an outpatient basis where surgical capabilities can be limited there is a need for alternative treatment options. In this study we aim to evaluate the use of amino acid buffered hypochlorite (AABH) as a chemical enhancement for wound debridement in a porcine infected burn wound model.MethodA total of 60 full-thickness burn wounds, 3 cm in diameter, were created on four pigs using a standardized burn device. The wounds were inoculated with 107 colony-forming units (CFU) of S. aureus. The experimental groups included wounds debrided with a plastic curette, wounds debrided after pretreatment with AABH, and control wounds wiped with gauze. Wounds were treated twice per week for three weeks. Debridement, healing, and infection parameters were evaluated over time.ResultsAfter one week, but not after two and three weeks, the curette and AABH groups had higher debrided weights compared to control (p < 0.05). Percentage of wound area adequately cleared from necrotic tissue was higher in the AABH-group compared to the curette-group and control, after one week. The earliest healing was measured in the AABH group after two weeks (5 % of wounds), which also had the most healed wounds after three weeks (55 %). In both the AABH and the curette groups, bacterial load had fallen below 105 CFU/g after two weeks. No CFU were detectable in the AABH group after three weeks. The AABH-group was also the easiest to debride.ConclusionOur results indicate that AABH facilitates wound debridement and could be a helpful addition to an effective treatment modality for removal of necrotic tissue in full-thickness burns.Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.

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