• World Neurosurg · Mar 2023

    Protective and Therapeutic Effects of Bovine Amniotic Fluids Collected in Different Trimesters on The Epidural Fibrosis After Experimental Laminectomy in Rats.

    • Ziya Yurtal, Kadri Kulualp, Huseyin Ozkan, Serap Cilaker Micili, Halef Dogan, Ali Riza Sisman, Nevin Ersoy, Ceren Kizmazoglu, and Akin Yakan.
    • Department of Surgery, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, Hatay, Turkey. Electronic address: ziyayurtal@gmail.com.
    • World Neurosurg. 2023 Mar 1; 171: e722e730e722-e730.

    BackgroundThe aim of this study was to investigate the protective and therapeutic effects of bovine amniotic fluid (BAF) on the inhibition of epidural fibrosis (EF) after experimental laminectomy.MethodsForty female Sprague Dawley rats were used. The amniotic fluids were collected from each trimester of a pregnant cow. The rats were divided into 5 groups. Whereas no laminectomy was applied to the control group, animals in the sham group underwent laminectomy. Laminectomy was performed in the animals in other groups and the operation area was closed by dripping 1 mL of BAF collected in 3 trimesters of pregnancy. Animals were killed 28 days after the operation.ResultsCompared with control, VEGF gene expression levels were downregulated approximately 5-fold in BAF-2. Whereas IL-6 was upregulated approximately 8-fold in the sham, it was downregulated 5-fold and 3-fold in BAF-1 and BAF-2, respectively. There was downregulation in BAF-2 and BAF-3 in terms of CD105 gene expression levels. TGFβ1 was upregulated approximately 2-fold in the sham group and downregulated in BAF-1 and BAF-2. Although histopathologic alterations including EF grade and fibroblast cell density were found to increase in the sham group, all BAF treatment decreased those of alterations. The highest CD105 immunoreactivity was detected in the sham group. All BAF treatment markedly aggravated fibrosis via decreasing CD105 immunoreactivity. In terms of grading parameters, almost the closest grades to the control were determined in the BAF-2. BAF collected in the second trimester is most effective in healing of scar tissue and preventing fibrosis via decreasing microvessel and fibroblast densities.ConclusionsThe results indicate that BAF may be used as a potential protective agent to prevent EF.Copyright © 2022 Elsevier Inc. All rights reserved.

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