• Ishor Pradhan, Subhash Regmi, Meena Kunwar, Bibek Basukala, and Amit Joshi.
• Department of Orthopedics, B & B Hospital, Gwarko, Lalitpur, Nepal.
• JNMA J Nepal Med Assoc. 2022 Aug 1; 60 (252): 667671667-671.

IntroductionA hospital-based investigation of bacteriological isolates helps to identify common causative bacteria and their antibiotic sensitivity patterns. This helps in formulating presumptive antibiotic therapy and in reducing antibiotic misuse. The aim of this study was to find out the prevalence of positive bacterial culture isolates among suspected orthopaedic infections in a tertiary care centre.MethodsA descriptive cross-sectional study was conducted from the electronic data record of the Department of Microbiology of a tertiary care centre from 1 January 2017 to 31 December 2021. The study was conducted following ethical approval from the Institutional Review Committee (Reference number: IRC-2021-11-09-1). Culture reports of suspected orthopaedic infections were evaluated, and those with missing data were excluded. A convenience sampling method was used. Point estimate and 95% Confidence Interval were calculated.ResultsOut of 6201 specimens, positive bacterial culture were found in 2957 (47.69%) (46.45-48.93, 95% Confidence Interval). Among them, 1561 (56.01%) were gram-negative organisms and 677 (24.29%) were gram-positive. A total of 2787 (94.25%) were wound/pus swab cultures and 170 (5.74%) were tissue cultures.ConclusionsThe prevalence of positive bacterial culture among suspected orthopaedic infections was lower than in other international studies. Among bacteriological isolates, gram-negative organisms are more than gram-positive organisms.Keywordsculture techniques; infections; microbial sensitivity tests; prevalence.

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