• Mehmet Suhha Bostancı, Ozcan Budak, Hüseyin Çakiroğlu, Osman Köse, Özkan Durmaz, and Erdem Cokluk.
• Sakarya Üniversitesi, Faculty of Medicine, Department of Obstetrics and Gynecology and Artificial Reproductive Techniques - Serdivan, Turkey.
• Rev Assoc Med Bras (1992). 2023 Jan 1; 69 (3): 380385380-385.

ObjectiveThe study aimed to investigate the protection of enoxaparin (E) against experimental ischemic (I) and ischemic-reperfusion (I/R) injury in rat ovaries on in vitro fertilization outcomes.MethodsIn total, 56 adult female Sprague-Dawley albino rats were randomly assigned to 6 groups of 8 animals each: Sham, Ischemia, I/R, Sham+E, I+E, and I/R+E. Ischemia groups were subjected to bilateral adnexal torsion for 3 h. In contrast, I/R and I/R+E groups received subsequent detorsion for 3 h. Enoxaparin (0.5 mg/kg s.c.) was administered 30 min prior to ischemia (I+platelet-rich plasma) or reperfusion (I/R+I+platelet-rich plasma). Ovaries were stimulated through intraperitoneal injection of 150-300 internal units IU/kg pregnant mare serum gonadotropin. Anti-Müllerian hormone levels were measured before and after surgery in all groups.ResultsWhen the number of metaphase II oocytes was evaluated, statistically significant differences were observed between the I and I+E (p=0.001) and I/R and I/R+E (p=0.000) groups. When both I and I+E groups and I/R and I/R+E groups were compared, it was found that E application increased the number of fertilized oocytes. The number of embryos on the second day was higher in the I/R+E group than that in the I/R group. Statistically significant differences were found in the number of grade 1 embryos between the I/R and I/R+E groups (p=0.003). In comparing anti-Müllerian hormone values within the group, the highest decrease was observed in the I and I/R groups.ConclusionEnoxaparin effectively minimizes ovarian damage and preserves ovarian reserve following ovarian torsion.

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