• Wenjie Cheng, Zhe Wu, Jizheng Zhang, and Wanlu Ren.
• Tianjin Hospital, Department of Anesthesiology - Tianjin, China.
• Rev Assoc Med Bras (1992). 2023 Jan 1; 69 (2): 228232228-232.

ObjectiveThe aim of this study was to investigate whether dexmedetomidine could reduce tourniquet-induced skeletal muscle injury.MethodsC57BL6 male mice were randomly assigned to sham, ischemia/reperfusion, and dexmedetomidine groups. Mice in the ischemia/reperfusion and dexmedetomidine groups received normal saline solution and dexmedetomidine intraperitoneally, respectively. The sham group underwent the same procedure as the ischemia/reperfusion group, with the exception of tourniquet application. Subsequently, the ultrastructure of the gastrocnemius muscle was observed, and its contractile force was examined. In addition, Toll-like receptor 4 and nuclear factor-κB expression within muscles was detected by Western blot.ResultsDexmedetomidine alleviated myocyte damage and increased the contractility of skeletal muscles. Moreover, dexmedetomidine significantly inhibited the expression of Toll-like receptor 4/nuclear factor-κB in the gastrocnemius muscle.ConclusionTaken together, these results demonstrate that dexmedetomidine administration attenuated tourniquet-induced structural and functional impairment of the skeletal muscle, partly through inactivation of the Toll-like receptor 4/nuclear factor-κB pathway.

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