• Bogdan Ciornei, Adrian Vaduva, Vlad Laurentiu David, Diana Popescu, Dan Dumitru Vulcanescu, Ovidiu Adam, Cecilia Roberta Avram, Alina Cornelia Pacurari, and Eugen Sorin Boia.
• Department of Pediatric Surgery and Orthopedics, "Victor Babes" University of Medicine and Pharmacy, 300002 Timisoara, Romania.
• Medicina (Kaunas). 2023 May 23; 59 (6).

AbstractBackground and Objectives: Skin scaffolding can be done using allografts and autografts. As a biological allograft, the skin of Oreochromis niloticus (ON) has been used due to its high type I and III collagen content. Oreochromis mossambicus (OM) is also a member of the Oreochromis family, but not much is known regarding its collagen content. As such, this study aimed to assess and compare the collagen content of the two fish species. Materials and Methods: This is a crossover study comparing the skin collagen contents of the two fish. Young fish were chosen, as they tend to have higher collagen concentrations. The skin samples were sterilized in chlorhexidine and increasing glycerol solutions and analyzed histochemically with Sirius red picrate under polarized light microscopy. Results: 6 young ON and 4 OM specimens were used. Baseline type I collagen was higher for OM, but at maximum sterilization it was higher for ON, with no differences in between Type III collagen was higher for OM across all comparisons with the exception of the last stage of sterilization. Generally, collagen concentrations were higher in highly sterilized samples. Conclusions: OM skin harvested from young fish, with its greater collagen III content may be a better candidate for use as a biological skin scaffold in the treatment of burn wounds, compared to ON.

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