• I Dimopoulou, S E Orfanos, A Pelekanou, A Kotanidou, O Livaditi, C Augustatou, M Zervou, E Douka, M Theodorakopoulou, V Karagianni, E Douzinas, A Armaganidis, and E J Giamarellos-Bourboulis.
• 2nd Department of Critical Care Medicine, University of Athens, Greece.
• Inflamm. Res. 2009 Mar 1;58(3):127-32.

ObjectivesTo describe the concentrations of sTREM-1 in patients with sepsis and to explore the effects of their serum on the expression of TREM-1 on U937 monocytes.MethodsBlood was sampled at regular time intervals in 56 patients with sepsis. Concentrations of tumour necrosis factor-alpha (TNFalpha), interleukin-1beta (IL-1alpha), IL-6, IL-8, IL-10 and IL-12p70 and sTREM-1 were measured. U937 monocytes were incubated in the presence of serum at sepsis onset.ResultsMedian sTREM-1 concentration on day 1 for patients with septic shock was 915 pg/ml and 228.5 pg/ml for those without shock (p = 0.002). TNFalpha, IL-1alpha, IL-6, IL-8 and IL-10 did not differ between them. A positive correlation was found between changes of sTREM-1 and SOFA scores from day 1 to 7. Sera of patients with septic shock evoked a significant increase of the expression of TREM-1. The concentrations of TNFalpha and IL-8 in supernatants increased only after stimulating with sera of patients without shock, but not after stimulating with sera of patients with shock.ConclusionsLevels of sTREM-1 correlated with sepsis severity. sTREM-1 is considerably higher in patients with shock compared to patients without shock. The serum of shocked patients could stimulate the expression of TREM-1 on U937 monocytes.

23 keywords...

hide…