• Carlos Eduardo Macedo, Adriano Menis Ferreira, Larissa da Silva Barcelos, André Luiz Silva Alvim, Liliane Moretti Carneiro, Sandro Rogério Martins, AndradeDenise deD0000-0002-3336-2695PhD. Nurse, Full Professor, Ribeirão Preto College of Nursing, Universidade de São Paulo (USP), Ribeirão Preto, São Paulo (SP), Brazil., Marcelo Alessandro Rigotti, Ruberval Peres Gasques, Vanderlei Amaro da Silva Junior, Layze Braz de Oliveira, Herica Emilia Félix de Carvalho, and Alvaro Francisco Lopes de Sousa.
• MD. Physician, Postgraduate Program in Nursing, Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande (MS), Brazil.
• Sao Paulo Med J. 2024 Jan 1; 142 (4): e2023177e2023177.

BackgroundContamination of the breathing circuit and medication preparation surface of an anesthesia machine can increase the risk of cross-infection.ObjectiveTo evaluate the contamination of the anesthetic medication preparation surface, respiratory circuits, and devices used in general anesthesia with assisted mechanical ventilation.Design And SettingCross-sectional, quantitative study conducted at the surgical center of a philanthropic hospital, of medium complexity located in the municipality of Três Lagoas, in the eastern region of the State of Mato Grosso do Sul.MethodsEighty-two microbiological samples were collected from the breathing circuits. After repeating the samples in different culture media, 328 analyses were performed.ResultsA higher occurrence of E. coli, Enterobacter spp., Pseudomonas spp., Staphylococcus aureus, and Streptococcus pneumoniae (P < 0.001) were observed. Variations were observed depending on the culture medium and sample collection site.ConclusionThe study findings underscore the inadequate disinfection of the inspiratory and expiratory branches, highlighting the importance of stringent cleaning and disinfection of high-touch surfaces.

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