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J. Korean Med. Sci. · Feb 2016
Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak.
- Wan Beom Park, Nak-Jung Kwon, Pyoeng Gyun Choe, Su-Jin Choi, Hong Sang Oh, Sang Min Lee, Hyonyong Chong, Jong-Il Kim, Kyoung-Ho Song, Ji Hwan Bang, Eu Suk Kim, Hong-Bin Kim, Sang Won Park, Nam Joong Kim, and Myoung-Don Oh.
- Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.; Laboratory of Infection & Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, Korea.
- J. Korean Med. Sci. 2016 Feb 1; 31 (2): 315320315-20.
AbstractDuring the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV.
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