• Mohammad Abdolvand, Zahra Mohammadi Chermahini, Sahar Bahaloo, Mohammad Hassan Emami, Alireza Fahim, Hojjatolah Rahimi, Elham Amjadi, Fatemeh Maghool, Fattah Rohani, Mina Dadkhah, Nooshin Farhadian, Nasimeh Vatandoust, Shirin Abdolvand, Maliheh Roozbahani Darehsari, Mohammad Chehelgerdi, Faeze Ahmadi Beni, Mahsa Khodadoostan, Simin Hemati, and Mansoor Salehi.
• Cellular, Molecular and Genetics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
• J Res Med Sci. 2024 Jan 1; 29: 1010.

BackgroundCancer development is aided by the role of long noncoding RNAs (lncRNAs) that act as competing endogenous RNAs (ceRNAs) absorbing microRNAs (miRNAs). We aimed to discover a novel regulatory axis in colorectal cancer (CRC) and potential biomarkers based on miR-616-3p.Materials And MethodsThe gene expression omnibus database was mined for differentially expressed lncRNAs (DELs) and mRNAs. LncRNAs and mRNAs were predicted using the RegRNA and TargetScan databases. A combination of the ciBioPortal and Ensemble databases was used to locate the mRNAs. Cytoscape 3.7.1-built CeRNA networks. A quantitative real-time polymerase chain reaction (qRT-PCR) was utilized to confirm the expression levels of these RNA molecules. Statistical analyses were implemented by GraphPad Prism 9.ResultsqRT-PCR showed (Linc01282, lnc-MYADM-1:1, and Zinc Finger Protein 347 [ZNF347]) were overexpressed whereas, (salt-inducible kinases 1 [SIK1], and miR-616-3p) were down regulated.ConclusionThese results identify unique, unreported lncRNAs as CRC prognostic biomarkers, as well as prospective mRNAs as new treatment targets and predictive biomarkers for CRC. In addition, our study uncovered unexplored ceRNA networks that should be studied further in CRC.Copyright: © 2024 Journal of Research in Medical Sciences.

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