• Mingzi Zhang, Shu Liu, Enling Guan, Hao Liu, Xinhang Dong, Yan Hao, Xin Zhang, Pengxiang Zhao, Xuehua Liu, Shuyi Pan, Youbin Wang, Xiaojun Wang, and Yifang Liu.
• Department of Plastic Surgery, Peking Union Medical College Hospital Department of Plastic Surgery, China Meitan General Hospital Affiliated to North China University of Science and Technology, Beijing Department of Ear-Nose-Throat, Qingdao Huangdao District Hospital of Traditional Chinese Medicine, Qingdao, Shandong College of Life Science and Bioengineering, Beijing University of Technology Department of Hyperbaric Oxygen, Beijing Chao-Yang Hospital Department of Hyperbaric Oxygen, Navy General Hospital International education college, Beijing Vocational College of Agriculture, Beijing, China.
• Medicine (Baltimore). 2018 Jul 1; 97 (29): e11529e11529.

BackgroundHyperbaric oxygen therapy (HBOT) has been widely used in the clinical setting. In this study, HBOT therapy was evaluated for its ability to ameliorate the epithelial-to-mesenchymal transition (EMT) phenomenon in keloid tissue.MethodsKeloid patients were randomly divided into two groups: keloid patients (K group, 9 patients) and keloid patients receiving HBOT (O group, 9 patients). A third group with normal skin (S group, 9 patients) was established for control. Before HBOT and surgery, a laser Doppler flowmeter was used to measure the keloid blood supply of patients in the O group. Hematoxylin and eosin (H&E) staining was used to observe morphology. E-cadherin, ZO-1, vimentin, fibronectin, vascular endothelial growth factor (VEGF), and hypoxia inducible factor (HIF)-1α were measured by immunofluorescence staining and Western blot analysis. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to evaluate the mRNA expression level of these factors as well.ResultsIn the O group, keloid blood perfusion was significantly reduced after patients received HBOT. Compared with the K group, lower expression levels of vimentin, vibronectin, VEGF, and HIF-1α were observed in the O group, whereas the expression of E-cadherin and ZO-1 was significantly higher. The mRNA expression of E-cadherin and ZO-1 was also increased after HBOT.ConclusionsThe expression levels of factors related to the EMT phenomenon were significantly reversed in keloid patients after they received HBOT, indicating that HBOT may be an effective therapy against the EMT phenomenon in keloid patients.

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