• Alexander Wisniewski, Anthony Norman, Siny Tsang, Robert Ricketts, Ruchik Sharma, James Suggs, Samantha Vizzini, Barbara Castro, Jacob Raphael, Peggy McNaull, James Gangemi, Mark Roeser, and Michael Mazzeffi.
• University of Virginia, Department of Surgery, Division of Cardiothoracic Surgery, Charlottesville, VA.
• J. Cardiothorac. Vasc. Anesth. 2024 Sep 1; 38 (9): 200920162009-2016.

ObjectivesThis work was designed to evaluate maximum platelet contractile force and thrombus area before and after cardiopulmonary bypass (CPB) in pediatric patients having congenital heart disease (CHD) surgery using a microfluidic device.DesignA prospective cohort study was designed.SettingThe work took place at an academic medical center.ParticipantsTwenty pediatric CHD patients ≤8 years of age with expected CPB time >30 minutes were enrolled.InterventionsNone.Measurements And Main ResultsBlood was collected at baseline and post-CPB. Maximum platelet contractile force and thrombus area were evaluated in vitro using a microfluidic device (ATLAS PST). Post-CPB samples were supplemented with recombinant von Willebrand factor (rVWF) to explore the impact on contractile force and thrombus area. At baseline, the maximum thrombus area was 0.06 (0.05, 0.07), and the maximum force was 123.3 nN (68.4, 299.5). Linear mixed-effects regression models showed that the maximum thrombus area was larger post-CPB and post-CPB + rVWF compared with pre-CPB (estimated coefficient [Est] = 0.04, p = 0.002; Est = 0.09, p < 0.001, respectively). The maximum thrombus area was also larger post-CPB + rVWF compared with post-CPB (Est = 0.04, p = 0.001). Force was higher post-CPB + rVWF compared with pre-CPB (Est = 173.32, p = 0.044).ConclusionsIn pediatric CHD patients, microfluidic testing demonstrated that platelet thrombus area increased slightly after CPB, while platelet contractile force did not change. In vitro addition of rVWF further increased thrombus area, suggesting augmentation of primary hemostasis. Microfluidic assessment of platelet contractile force and thrombus area in pediatric CHD patients appears feasible and can demonstrate changes after CPB. Further studies are needed to determine its accuracy, clinical utility, and normal values for pediatric patients.Copyright © 2024 Elsevier Inc. All rights reserved.

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