• Yanhua Shi, Housheng Deng, Zhiming Zhang, Xiaoling Zhu, and Zhiqin Zeng.
• Department of Anesthesiology, Chenzhou First People's Hospital, No.102 Luojiajing, Beihu District, Chenzhou City, 423000, Hunan Province, People's Republic of China.
• BMC Anesthesiol. 2024 Jul 25; 24 (1): 251251.

BackgroundIschemia-reperfusion (I/R) injury is a major factor in liver damage following hepatic resection and liver transplantation, with anesthetics demonstrating the ability to shield organs from this type of injury.MethodsHypoxia-reoxygenation (H/R) was used to create in vitro I/R hepatocyte cell injury models. The CCK-8 assay, flow cytometer, LDH assay, and ELSIA were utilized to assess hepatocyte injury. The in vivo I/R injury rat model was then built. HE and TUNEL staining were used to assess liver tissue damage. Western-blot was applied to assess the activation of the MAPK/ERK pathway.ResultsRemimazolam (RMZL) remarkably improved cell viability and decreased apoptosis in H/R-induced hepatocyte injury. RMZL reduced the release of H/R-induced inflammatory mediators (TNF-α and IL-6) as well as LDH levels. We also discovered that RMZL inhibited p38 and ERK1/2 phosphorylation in vivo and in vitro. The stimulation of MAPK/ERK, on the other hand, abolished RMZL's anti-inflammation effects in H/R-induced hepatocyte injury. Furthermore, RMZL reduced liver tissue injury in I/R rats.ConclusionRMZL prevented hepatic I/R damage by inhibiting MAPK/ERK signaling.© 2024. The Author(s).

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