• Angeliki Cheva, Angeliki Chorti, Kassiani Boulogeorgou, Anthoula Chatzikyriakidou, Charoula Achilla, Vangelis Bontinis, Alkis Bontinis, Stefanos Milias, Thomas Zarampoukas, Sohail Y Bakkar, and Theodosios Papavramidis.
• Laboratory of Pathology, Faculty of Health Science, Medical School, Aristotle University, 541 24 Thessaloniki, Greece.
• Medicina (Kaunas). 2024 Jul 5; 60 (7).

AbstractBackground and Objectives: Parathyroid adenoma is a distinct cause of primary hyperparathyroidism, with the vast majority being sporadic ones. Proteomic analysis of parathyroid adenomas has proposed a large number of related proteins. The aim of this study is to evaluate the immunohistochemical staining of ANXA2, MED12, MAPK1 and VDR in parathyroid adenoma tissue. Materials and Methods: Fifty-one parathyroid adenomas were analyzed for ANXA2, MED12, MAPK1 and VDR expressions. Tissue was extracted from formalin-fixed paraffin-embedded parathyroid adenoma specimens; an immunohistochemical study was applied, and the percentage of allocation and intensity were evaluated. Results: ANXA2 stained positively in 60.8% of all cell types, while MED12 had positive staining in 66%. MAPK1 expression was found to be negative in total, although a specific pattern for oxyphil cells was observed, as they stained positive in 17.7%. Finally, VDR staining was positive at 22.8%, based on nuclear staining. Conclusions: These immunohistochemical results could be utilized as biomarkers for the diagnosis of sporadic parathyroid adenoma. It is of great importance that a distinct immunophenotype of nodule-forming cells in a positive adenoma could suggest a specific pattern of adenoma development, as in hereditary patterns.

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